GK079 ScienCell 人類軟骨細(xì)胞生物學(xué)qPCR檢測試劑盒，Human Chondrocyte Biology qPCR Array Kit

ScienCell 研究實(shí)驗室（ScienCell）是一家生物技術(shù)公司，其使命是實(shí)驗和治療用細(xì)胞和細(xì)胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動物細(xì)胞，細(xì)胞培養(yǎng)基和試劑，培養(yǎng)基添加因子，細(xì)胞來源的RNA，cDNA和蛋白質(zhì)。ScienCell的科學(xué)家們以改善生活質(zhì)量，實(shí)現(xiàn)細(xì)胞的能力，打擊人類退化性疾病為目的研究和開發(fā)各種細(xì)胞治療策略。ScienCell總部設(shè)在美國加利福尼亞州圣地亞哥，公司成立于1999年。

北京諾博萊德作為ScienCell品牌的經(jīng)銷商，為客戶提供，正規(guī)進(jìn)口，貨期穩(wěn)定，折扣好的服務(wù)標(biāo)準(zhǔn)。

GK079 ScienCell人類軟骨細(xì)胞生物學(xué)qPCR檢測試劑盒，Human Chondrocyte Biology qPCR Array Kit，產(chǎn)品簡介：

產(chǎn)品品牌： ScienCell

產(chǎn)品貨號：GK079

產(chǎn)品名稱：GK079 ScienCell人類軟骨細(xì)胞生物學(xué)qPCR檢測試劑盒，Human Chondrocyte Biology qPCR Array Kit

產(chǎn)品規(guī)格：One 96-well plate, one 20ul reaction per well

GK079 ScienCell人類軟骨細(xì)胞生物學(xué)qPCR檢測試劑盒，Human Chondrocyte Biology qPCR Array Kit， 產(chǎn)品說明：

ScienCell 的 GeneQuery™ 人軟骨細(xì)胞生物學(xué) qPCR 芯片試劑盒 （GQH-CHN） 有助于對軟骨細(xì)胞功能中的 88 個關(guān)鍵基因進(jìn)行基因表達(dá)分析。 軟骨細(xì)胞嵌入軟骨基質(zhì)中，負(fù)責(zé)產(chǎn)生和維持軟骨基質(zhì)。 軟骨細(xì)胞起源于間充質(zhì)，在骨化過程中終末分化時肥大。 如何根據(jù)其在軟骨細(xì)胞生物學(xué)中的功能對所包含的基因進(jìn)行分組的簡要示例如下所示：

軟骨生成： COL2A1， ACAN， SOX9， MIA， PTCH1

ECM相互作用：RHOA，ITGAV，CD44，PTK2，ITGB1

機(jī)械轉(zhuǎn)導(dǎo)： NFATC4， TRPV4， CREB1， MAPK1， PRKCE

改造和修復(fù)：BMP2，HIF1A，MMP9，WNT9A，ICAM1

GeneQuery™ qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒，每個孔包含一個引物組，可識別并有效擴(kuò)增特定靶基因的 cDNA。精心設(shè)計的引物確保：（i）qPCR分析中的優(yōu)良退火溫度為65°C（含2 mM Mg2+且不含DMSO）;（ii）除非另有說明，否則引物集可識別靶基因的所有已知轉(zhuǎn)錄本變體;（iii）只有一個基因被擴(kuò)增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進(jìn)行了驗證。

ScienCell's GeneQuery™ Human Chondrocyte Biology qPCR Array Kit (GQH-CHN) facilitates gene expression profiling of 88 key genes in chondrocyte function.  Chondrocytes are embedded in the cartilage matrix and are responsible for producing and maintaining it.  Chondrocytes originate in the mesenchyme and are hypertrophic when terminally differentiated during ossification.  Brief examples of how included genes may be grouped according to their function in chondrocyte biology are shown below:

·         Chondrogenesis: COL2A1, ACAN, SOX9, MIA, PTCH1

·         ECM interaction: RHOA, ITGAV, CD44, PTK2, ITGB1

·         Mechanotransduction: NFATC4, TRPV4, CREB1, MAPK1, PRKCE

·         Remodeling and repair: BMP2, HIF1A, MMP9, WNT9A, ICAM1

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

基因查詢™人軟骨細(xì)胞生物學(xué) qPCR 陣列板布局*（8 個粗體和斜體對照）

提示：基因選擇可能會根據(jù)新的研究和開發(fā)進(jìn)行更新

ScienCell基因表達(dá)分析產(chǎn)品訂購信息：

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GK002   人類多能干細(xì)胞qPCR檢測試劑盒，Human Pluripotent Stem Cell qPCR Array Kit

GK003   人類細(xì)胞周期qPCR檢測試劑盒，Human Cell Cycle qPCR Array Kit

GK005   人上皮細(xì)胞生物學(xué)qPCR檢測試劑盒，Human Epithelial Cell Biology qPCR Array Kit

GK006   人類神經(jīng)發(fā)育和再生qPCR檢測試劑盒，Human Neural Development and Regeneration qPCR Array Kit

GK007   人類神經(jīng)可塑性qPCR檢測試劑盒，Human Neural Plasticity qPCR Array Kit

GK008   人類神經(jīng)傳遞和膜轉(zhuǎn)運(yùn)qPCR檢測試劑盒，Human Neural Transmission and Membrane Trafficking qPCR Array Kit

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GK010   人類黑素細(xì)胞發(fā)育和色素沉著qPCR檢測試劑盒，Human Melanocyte Development & Pigmentation qPCR Array Kit

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GK018   人內(nèi)皮細(xì)胞表型異質(zhì)性qPCR檢測試劑盒，Human Endothelial Cell Phenotypic Heterogeneity qPCR Array Kit

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GK021   人類T細(xì)胞受體信號通路qPCR檢測試劑盒，Human T Cell Receptor Signaling Pathway qPCR Array Kit

GK022   人內(nèi)吞作用qPCR檢測試劑盒，Human Endocytosis qPCR Array Kit

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GK026   人類Wnt信號通路qPCR檢測試劑盒，Human Wnt Signaling Pathway qPCR Array Kit

GK027   人類非經(jīng)典Wnt信號通路qPCR檢測試劑盒，Human Noncanonical Wnt Signaling Pathways qPCR Array Kit

GK028   人類Wnt信號靶基因qPCR檢測試劑盒，Human Wnt Signaling Target Genes qPCR Array Kit

GK029   Human Tie2/Angpt信號qPCR檢測試劑盒，Human Tie2/Angpt Signaling qPCR Array Kit

GK034   人肺纖維化qPCR檢測試劑盒，Human Pulmonary Fibrosis qPCR Array Kit

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GK041   人體皮膚傷口愈合qPCR檢測試劑盒，Human Skin Wound Healing qPCR Array Kit

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GK061   人類DNA損傷感應(yīng)qPCR檢測試劑盒，Human DNA Damage Sensing qPCR Array Kit

GK062   人類細(xì)胞生長和分裂qPCR檢測試劑盒，Human Cell Growth and Division qPCR Array Kit

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GK074   人角質(zhì)細(xì)胞生物學(xué)qPCR檢測試劑盒，Human Keratocyte Biology qPCR Array

GK078   人類神經(jīng)細(xì)胞生物學(xué)qPCR檢測試劑盒，Human Neuronal Cell Biology qPCR Array Kit

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GK090   人體細(xì)胞連接qPCR檢測試劑盒，Human Cell Junctions qPCR Array Kit

GK091   人肝星狀細(xì)胞生物學(xué)qPCR檢測試劑盒，Human Hepatic Stellate Cell Biology qPCR Array Kit

GK092   人胰腺星狀細(xì)胞生物學(xué)qPCR檢測試劑盒，Human Pancreatic Stellate Cell Biology qPCR Array Kit

GK093   Human Renal Mesangial Cell Biology qPCR Array Kit，Human Renal Mesangial Cell Biology qPCR Array Kit

GK094   Human Mesenchymal Stem Cell Differentiation qPCR Array，Human Mesenchymal Stem Cell Differentiation qPCR Array Kit

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GK097   人類平滑肌細(xì)胞生物學(xué)qPCR檢測試劑盒，Human Smooth Muscle Cell Biology qPCR Array Kit

GK098   人類心臟肌細(xì)胞生物學(xué)qPCR檢測試劑盒，Human Cardiac Myocyte Biology qPCR Array Kit

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GK100   人角質(zhì)形成細(xì)胞生物學(xué)qPCR檢測試劑盒，Human Keratinocyte Cell Biology qPCR Array Kit

GK101   人類Synoviocyte細(xì)胞生物學(xué)qPCR檢測試劑盒，Human Synoviocyte Cell Biology qPCR Array Kit

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GK105   人類ECM降解qPCR檢測試劑盒，Human ECM Degradation qPCR Array Kit

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GK113   人類神經(jīng)干細(xì)胞標(biāo)記qPCR檢測試劑盒，Human Neural Stem Cell Markers qPCR Array Kit

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GK115   人類骨髓譜系標(biāo)記qPCR檢測試劑盒，Human Myeloid Lineage Markers qPCR Array Kit

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GK124   人類轉(zhuǎn)錄因子qPCR檢測試劑盒，Human Transcription Factors qPCR Array Kit

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GK126   人類細(xì)胞表面標(biāo)記qPCR檢測試劑盒，Human Cell Surface Markers qPCR Array Kit

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